2026-10-03 · 原文发表于 2026-09-23
双层PCL/GelMA微针贴片在糖尿病大鼠中改善创面修复指标
Sepanjnia A, Farzin A, Rezaei A, Seyhoon I, Arab M, Verdi J.. Bilayer PCL/GelMA microneedle patch codelivers deferoxamine and primed mesenchymal stem cell secretome for enhanced diabetic wound repair. Europe PMC · 创面修复文献. 2026.
查看原始文献（Europe PMC · 创面修复文献）↗研究问题
摘要指出，慢性糖尿病创面通常伴有持续性炎症、再上皮化受损、细胞外基质调节异常和微血管功能障碍，这些因素会妨碍创面愈合。研究因此构建了一个兼具材料支撑和局部递送功能的微针平台。摘要未提供更具体的既往临床证据或现有治疗比较背景。
研究设计
主要发现
如何理解
局限与不确定性
一句话带走
在糖尿病大鼠创面模型中，DFO与CM共递送的双层PCL/GelMA微针贴片与更好的多项修复指标相关，结果支持其作为进一步研究的候选递送平台。现有摘要不足以证明其在人类糖尿病创面中的疗效、安全性或真实的协同机制。
术语小注
- PCL/GelMA：
- PCL是具有结构支撑作用的可降解聚合物；GelMA是明胶改性材料，可形成水凝胶并承载生物活性成分。
- 微针贴片：
- 带有微小针状结构的局部递送装置，可将材料或药物送入创面及其浅层组织。
- 去铁胺（DFO）：
- 一种铁螯合剂；本研究将其作为微针递送的活性成分，但摘要未详细说明其在该模型中的具体作用通路。
- 间充质干细胞条件培养基（CM）：
- 培养间充质干细胞后收集的培养液，含有细胞分泌的可溶性因子；摘要未说明其具体成分。
- 再上皮化：
- 创面表面重新形成上皮覆盖的过程，是创面闭合和屏障恢复的重要环节。
- CD31：
- 常用于标记血管内皮细胞的指标；本研究以CD31阳性血管结构评估血管相关变化。
展开原文摘要
Chronic diabetic wounds remain refractory to healing due to persistent inflammation, impaired re-epithelialization, extracellular matrix dysregulation, and microvascular dysfunction. Here, we report a mechanically robust and biologically integrated microneedle (MN) platform composed of a poly(ε-caprolactone) (PCL) core and a gelatin methacryloyl (GelMA) coating, engineered for the localized compartmentalized co-delivery of deferoxamine (DFO) and mesenchymal stem cell-derived conditioned medium (CM). By leveraging a dual-reservoir architecture-incorporating fresh liquid CM within the GelMA shell and lyophilized CM within the structural PCL core-the platform achieves complementary biphasic release kinetics while preserving the biological integrity of labile growth factors. In a diabetic wound model using Wistar rats, treatment with a bioactive MN platform significantly accelerated re-epithelialization, resolved chronic inflammation, and increased collagen deposition by approximately 40% compared to untreated and blank MN controls. Furthermore, vascular assessment on day 14 revealed a qualitative increase in CD31-positive vascular structures within the treated wounds. Notably, microneedles co-loaded with DFO and CM markedly improved key healing parameters compared with untreated diabetic wounds, with several parameters approaching those observed in non-diabetic control tissues at the 14-day endpoint. Mechanistically, these findings are consistent with a hierarchical healing process in which restoration of immune homeostasis promotes epithelialization and extracellular matrix remodeling, followed by enhanced vascularization. Collectively, these results indicate that spatially compartmentalized MN-mediated co-delivery of DFO and CM may represent a promising strategy for activating endogenous regenerative pathways and enhancing tissue repair in diabetic wounds. This approach may also provide a scalable therapeutic platform for improving the impaired healing capacity assoc
本站中文摘要：该研究在糖尿病Wistar大鼠创面模型中评估由聚ε-己内酯（PCL）核心和明胶甲基丙烯酰（GelMA）涂层组成的双层微针平台，用于局部共递送去铁胺（DFO）和间充质干细胞条件培养基（CM）。