2026-10-07 · 原文发表于 2026-09-29
富集miR-125b细胞外囊泡分体式微针对氮芥皮肤损伤显示前临床潜力
Jiang W, Meng Q, Liu K, Li A, Jin C, Zhu B, Zhu J, Wu M, Xu Q, Xue C.. Split-Type Microneedles Loaded with miR-125b-Enriched Extracellular Vesicles: A Sustained Preventive and Therapeutic Platform for Nitrogen Mustard-Induced Skin Injuries. ACS applied materials & interfaces. 2026. doi:10.1021/acsami.6c12383. PMID:42804766.
查看原始文献（Europe PMC · 创面修复文献）↗研究问题
摘要指出，氮芥可造成严重且难治的皮肤损伤，目前缺乏特异性治疗方法。细胞外囊泡在创面修复中显示出潜在作用，因此研究者进一步将miR-125b富集于工程化细胞外囊泡，并结合微针递送。
研究设计
主要发现
如何理解
局限与不确定性
一句话带走
在该前临床研究中，富集miR-125b细胞外囊泡的分体式微针表现出持续递送并减轻氮芥皮肤损伤的潜力。现有摘要证据尚不足以支持人体疗效或临床应用结论，仍需更完整的动物研究和临床验证。
术语小注
- 细胞外囊泡（EVs）：
- 细胞释放的膜性微小囊泡，可携带核酸和蛋白，并参与细胞间信息传递。
- miR-125b：
- 一种微小RNA，可调节多个基因的表达；本研究将其富集到细胞外囊泡中用于递送。
- 分体式微针：
- 由针尖和基底等部分组成的微针系统，可将药物或生物活性物质局部送入皮肤。
- 氮芥皮肤损伤：
- 氮芥暴露造成的皮肤损害，可表现为水疱、坏死和难以修复的创面。
- 血管生成：
- 形成新血管的过程，通常有助于为修复中的组织提供氧和营养。
展开原文摘要
Nitrogen mustard (NM) induces severe, refractory skin injuries lacking specific available therapeutics. Considering that extracellular vesicles (EVs) show promising effects in wound healing, we developed a split-type microneedle (MN-EVs@miR) loaded with miR-125b-enriched engineered EVs (EVs@miR) to treat NM-induced skin injury. EVs were isolated from human umbilical cord mesenchymal stem cells (hUMSCs), loaded with miR-125b via transfection, and then conjugated to hyaluronic acid methacryloyl (HAMA) hydrogel using acrylic acid-polyethylene glycol-N-hydroxysuccinimide (AC-PEG-NHS) to prepare microneedle tips, with polyvinyl alcohol (PVA) and sodium thiosulfate constituting the substrate. The prepared MN-EVs@miR exhibited sufficient mechanical strength (0.34 N/needle), sustained EVs@miR release over 21 days, and long-term in vivo retention (>21 days) with excellent biocompatibility. In vitro, EVs@miR enhanced cell viability, inhibited apoptosis, regulated inflammation (downregulating TNF-α, IL-1β, and IL-6; upregulating IL-10), and promoted angiogenesis. In vivo, MN-EVs@miR significantly mitigated NM-induced skin blistering and necrosis, with superior wound healing efficacy compared to that of EVs@miR injection and blank MNs. In conclusion, the split-type MN-EVs@miR provides a promising preventive and therapeutic strategy for NM-induced skin injuries with potential battlefield and clinical applications.
本站中文摘要：这项体外与体内前临床研究开发了负载富集miR-125b工程化细胞外囊泡的分体式微针（MN-EVs@miR），体内实验显示其显著减轻氮芥诱导的皮肤水疱和坏死，创面愈合效果优于囊泡注射及空白微针。