2026-10-10 · 原文发表于 2026-10-01
缺氧耐受性不同的大鼠在第11天呈现不同的创面修复特征
Dzhalilova DS, Tsvetkov IS, Kirillova MV, Melnikova EA, Mkhitarov VA, Diatroptova MA, Makarova OV.. Morphological and Molecular-Biological Characteristics of Wound Healing in Animals with High and Low Hypoxia Tolerance. Bulletin of experimental biology and medicine. 2026. doi:10.1007/s10517-026-06763-1. PMID:42821219.
查看原始文献（Europe PMC · 创面修复文献）↗研究问题
摘要显示，研究关注动物缺氧耐受性差异在创面修复研究中的影响。由于不同动物的缺氧耐受性可能与修复过程中的组织和分子反应相关，作者对两类大鼠进行了比较。摘要未进一步说明既有研究背景、临床意义或缺氧耐受性分型的具体方法。
研究设计
主要发现
如何理解
局限与不确定性
一句话带走
在该大鼠模型中，高缺氧耐受性与第11天较小的创面面积相关，低缺氧耐受性则伴随更高的Il6、Tgfb表达和血清IL-6水平。结果可用于提示动物缺氧耐受性可能影响创面修复研究结果，但尚不足以建立因果关系或外推为人体疗效。
术语小注
- 缺氧耐受性：
- 动物在低氧条件下维持生理功能和存活的能力；本研究将大鼠分为高耐受性和低耐受性两类。
- 全层皮肤创面：
- 损伤深度贯穿皮肤全层的实验性创面，通常用于观察完整的组织修复过程。
- 肉芽组织：
- 创面修复过程中形成的富含新生血管、成纤维细胞和细胞外基质的组织。
- PCR：
- 用于检测特定基因表达水平的分子生物学方法。
- ELISA：
- 用于测量血清或其他样本中特定蛋白浓度的免疫学检测方法。
- IL-6：
- 一种参与炎症反应和组织修复调控的细胞因子；本研究检测了其基因表达和血清蛋白水平。
展开原文摘要
The morphological and molecular genetic characteristics of wound healing were investigated in male Wistar rats with high and low hypoxia tolerance (HT and HS rats, respectively). A full-thickness skin wound (diameter 16 mm) was inflicted in the interscapular region. On day 11 after wound infliction (proliferation phase), histological examination and morphometric analysis of the wound tissue were performed. The expression of the Hif1a, Epas1, Vegf, Nfkb, Il1b, Il6, Il10, Tnfa, and Tgfb genes in intact skin and in the wound granulation tissue was assessed by PCR, and the serum levels of HIF-1α, IL-1β, IL-6, and IL-10 were detected by ELISA. On day 11 of wound healing, reparative processes were more pronounced in HT animals, manifesting as a significantly smaller wound area compared to HS rats. The ratio of collagen fiber types on day 11 of wound healing in HT and HS rats did not differ significantly. Compared to HT rats, HS animals demonstrated higher expression of the Il6 and Tgfb genes in the granulation tissue, and higher serum levels of IL-6 protein. These data should be considered when conducting studies using animals with different hypoxia tolerance.
本站中文摘要：该研究在高缺氧耐受性（HT）和低缺氧耐受性（HS）雄性Wistar大鼠背部建立直径16 mm的全层皮肤创面，并于伤后第11天评估组织形态、创面面积、相关基因表达及血清蛋白水平。