2026-10-12 · 原文发表于 2026-09-25
杏仁胶介导的银/氧化锌纳米复合材料在兔切除性创面模型中促进创面收缩
Rasool A, Shahid M, Mushtaq Z, Akhtar B.. Molecular docking-guided identification of multi-target anti-inflammatory phytochemicals from almond (Prunus dulcis) gum and experimental validation of almond gum-mediated silver/zinc oxide nanobiocomposites for accelerated wound healing. Europe PMC · 创面修复文献. 2026.
查看原始文献（Europe PMC · 创面修复文献）↗研究问题
摘要指出，失控的炎症可能延迟组织修复，因此研究者关注同时具有抗炎、抗菌和促修复潜力的杏仁胶纳米生物复合材料。该研究还强调，这是在同一杏仁胶基质中比较银纳米颗粒与氧化锌纳米颗粒的研究。摘要未提供更详细的临床背景或既有证据综述。
研究设计
主要发现
如何理解
局限与不确定性
一句话带走
在所报告的兔切除性创面模型中，杏仁胶介导的银和氧化锌纳米复合材料与较高创面收缩率及炎症指标调节相关，提示其具有进一步研究价值。但现有摘要证据主要来自计算、体外和动物实验，不能据此证明对人体创面有效或安全。
术语小注
- 分子对接：
- 用计算方法预测小分子与蛋白靶点的结合方式和相对结合倾向，属于机制假设工具，不等同于体内药效证据。
- ADME：
- 吸收、分布、代谢和排泄的统称，用于初步评估候选化合物的药代动力学特征。
- 纳米生物复合材料：
- 由纳米材料与生物来源基质组合形成的材料；本研究中的基质为杏仁胶。
- DPPH IC50：
- 在DPPH自由基清除实验中使反应指标达到一半抑制所需的浓度，数值越低通常表示体外自由基清除能力越强。
- 创面收缩率：
- 创面面积相对初始面积减少的比例，是动物创面修复研究中常用的观察指标，但不等同于完整、功能良好的皮肤再生。
- 溶血毒性：
- 材料导致红细胞破裂的潜在能力；摘要所称溶血毒性较低，仅反映所进行的体外测试结果。
展开原文摘要
Wound healing is a complex biological process where uncontrolled inflammation often delays tissue repair, imposing a significant clinical and economic burden worldwide. This study aimed to develop and evaluate almond gum (Prunus dulcis) based silver and zinc oxide nanobiocomposites (AG-Ag and AG-ZnO) through integrated computational, In-vitro and In-vivo studies as a promising therapeutic approach for accelerating wound healing. This study is the first to comparatively evaluate Ag-NPs and ZnO-NPs within the same almond gum matrix. A total of 31 phytoconstituents of Prunus dulcis were screened for ADME properties via pkCSM, and potential bioactive compounds were docked against COX-2, TNF-α, and VEGFR-2. Quercetin-3-diglucoside and Corosolic acid recorded the strongest binding affinities toward COX-2 (- 8.8 kcal/mol), mechanistically supporting the anti-inflammatory basis of Prunus dulcis. The green-synthesized nanobiocomposites were characterized via UV-Vis spectroscopy, FTIR, XRD, SEM, zeta sizer, and zeta potential, with mean particle sizes of 73.33 nm and 225.2 nm for AG-Ag and AG-ZnO, respectively. In-vitro studies revealed strong antioxidant activity, with AG-ZnO exhibiting the highest TPC (342.29 ± 1.18 µg GAE/g) and TFC (158.20 ± 0.40 µg CE/g), and AG-P the lowest DPPH IC50 (47.67 ± 2.01 µg/mL). Both nanobiocomposites further demonstrated potent antibacterial and anti-biofilm activities against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus subtilis, alongside significant anti-inflammatory activity and negligible hemolytic toxicity. In vivo evaluation using a rabbit excisional wound model demonstrated superior wound contraction with AG-Ag (90%) and AG-ZnO (93%) compared to the standard drug Polyfax (68%) and untreated control groups. Treatment groups also showed significant modulation of inflammatory biomarkers (ESR, CRP, IL-6) at Days 7 and 15 compared to controls. Histopathological studies showed active collagen deposition, fibr
本站中文摘要：该研究通过分子对接、材料表征、体外实验和兔切除性创面模型评价杏仁胶介导的银（AG-Ag）和氧化锌（AG-ZnO）纳米生物复合材料。31种杏仁（Prunus dulcis）植物化学成分接受ADME筛选，并针对COX-2、TNF-α和VEGFR-2进行对接；AG-Ag和AG-ZnO的平均粒径分别为73.33 nm和225.2 nm。