2026-10-29 · 原文发表于 2020-01-08
离体人皮肤模型再现再上皮化与免疫细胞行为
Rakita A, Nikolić N, Mildner M, Matiasek J, Elbe-Bürger A.. Re-epithelialization and immune cell behaviour in an ex vivo human skin model. Scientific reports. 2020;10(1):1. doi:10.1038/s41598-019-56847-4. PMID:31913322.
查看原始文献（Europe PMC · 创面修复近十年核心候选归档）↗研究问题
已有大量关于人体伤口愈合的研究，但缺乏一种能够维持真皮完整性、适用于标准化实验的离体创面模型。该研究旨在开发并验证一种基于负压作用的新型离体皮肤创面模型。
研究设计
主要发现
如何理解
局限与不确定性
一句话带走
该离体皮肤模型可作为研究表皮再生和免疫细胞行为的工具，适用于伤口愈合机制研究和治疗评估。但其结果不能直接用于指导个体临床治疗。
术语小注
- 再上皮化：
- 伤口愈合过程中，表皮细胞从边缘向中心迁移并增殖，覆盖创面的过程。
- 角质形成细胞：
- 皮肤表皮中主要的细胞类型，负责形成角质层，参与皮肤屏障功能。
- 免疫细胞：
- 参与机体免疫反应的细胞，包括T细胞、巨噬细胞等，可影响伤口愈合过程。
- 负压：
- 通过负压装置对创面施加的物理力，常用于促进伤口愈合。
- 基底膜：
- 位于表皮与真皮之间的结构，维持表皮细胞的正常定位和功能。
- 表皮分化标志物：
- 用于识别表皮不同分化阶段的分子标记，如K10、filaggrin等。
展开原文摘要
A large body of literature is available on wound healing in humans. Nonetheless, a standardized ex vivo wound model without disruption of the dermal compartment has not been put forward with compelling justification. Here, we present a novel wound model based on application of negative pressure and its effects for epidermal regeneration and immune cell behaviour. Importantly, the basement membrane remained intact after blister roof removal and keratinocytes were absent in the wounded area. Upon six days of culture, the wound was covered with one to three-cell thick K14 + Ki67 + keratinocyte layers, indicating that proliferation and migration were involved in wound closure. After eight to twelve days, a multi-layered epidermis was formed expressing epidermal differentiation markers (K10, filaggrin, DSG-1, CDSN). Investigations about immune cell-specific manners revealed more T cells in the blister roof epidermis compared to normal epidermis. We identified several cell populations in blister roof epidermis and suction blister fluid that are absent in normal epidermis which correlated with their decrease in the dermis, indicating a dermal efflux upon negative pressure. Together, our model recapitulates the main features of epithelial wound regeneration, and can be applied for testing wound healing therapies and investigating underlying mechanisms.
本站中文摘要：这项离体人皮肤研究建立了负压诱导的创面模型，在去除水疱顶壁后保留完整基底膜，观察到表皮再生及免疫细胞分布变化，属于前临床模型研究。培养6天后，创面被1至3层K14、Ki67阳性角质形成细胞覆盖，提示增殖与迁移参与创面闭合；8至12天后形成表达K10、filaggrin、DSG-1和CDSN的多层表皮。